Yimaphi amadayi asetshenziswa kakhulu ekusikeni izinto eziphilayo?

Kunezinhlobo ezahlukene zamadayi asetshenziswa kakhulu ekusikeni i-bioslicing, ngalinye lawo linezakhiwo ezahlukene kanye nobubanzi bokusetshenziswa. Nazi ezinye zamadayi asetshenziswa kakhulu ekusikeni i-bioslicing kanye nesingeniso sawo esifushane:

Okokuqala, udayi wemvelo

I-Hematoxylin: Lena umbala okhishwe emagatsheni omisiwe e-hematoxylum yaseNingizimu Melika (i-legume yasezindaweni ezishisayo) ngokuyifaka ku-ether. I-Hematoxylin ayikwazi ukudaywa ngqo, futhi idinga ukudaywa ukuze ibe yi-oxyhematoxylin (ebizwa nangokuthi i-hematoxylin) ngaphambi kokuba isetshenziswe. Iyinto enhle yokufaka idayi ku-nucleus futhi ingahlukanisa izakhiwo ezahlukene kuseli zibe imibala ehlukahlukene.

I-Carmine: I-Carmine, eyaziwa nangokuthi i-carmine noma i-carmine, yenziwe ngamabhungane e-cochineal ezinsikazi zasezindaweni ezishisayo omisiwe futhi agaywe abe yimpuphu, akhishwe obomvu wezinambuzane, bese ephathwa nge-alum ukuze ayenze. I-Carmagenta nayo iyidayi elihle le-nucleus, futhi isampula edaywe akulula ukuyifiphaza, ikakhulukazi ekudayiseni konke kwezinto ezincane.

Okwesibili, udayi wokwenziwa

I-Acid fuchsin: I-Acid fuchsin iyidayi ene-acid, impuphu ebomvu, encibilikayo emanzini, encibilikayo kancane otshwaleni. Iyi-ejenti enhle yokupenda amaseli, esetshenziswa kabanzi ekulungiseleleni izilwane, ekulungiseleleni izitshalo zesikhumba, i-pulp kanye namanye amaseli e-parenchyma kanye nezindonga ze-cellulose.

Ubomvu baseCongo: Ubomvu baseCongo buwudayi one-asidi, ngesimo sempuphu ebomvu ye-jujube, encibilika emanzini nasetshwaleni, oluhlaza okwesibhakabhaka ku-asidi. Ivame ukusetshenziswa ekukhiqizweni kwezitshalo njenge-liner ye-hematoxylin noma amanye amadayi eseli, futhi ingasetshenziswa futhi ukudaya i-cytoplasm kanye ne-nerve axes.

Okuluhlaza okotshani: Okuluhlaza okotshani kuyidayi ene-asidi, encibilikayo emanzini nasetshwaleni. Kuluhlobo lwe-ejenti yokudaya izicubu zeseli le-cellulose eziqukethe i-plasma, esetshenziswa kabanzi kumaseli okudaya nezicubu zezitshalo.

I-Sudan III: I-Sudan III iyidayi ye-asidi ebuthakathaka, i-powder ebomvu, encibilikayo emafutheni nasetshwaleni. Kuyibala lamafutha elivame ukusetshenziswa ukukhombisa okuqukethwe kwamafutha ezicutshini.

I-Eosin: Kunezinhlobo eziningi ze-eosin, futhi i-eosin Y evame ukusetshenziswa kakhulu iyidayi ene-asidi, ebomvu enamakristalu amancane aluhlaza okwesibhakabhaka noma impuphu ensundu. I-Eosin isetshenziswa kabanzi ekulungiseleleni izilwane, iyidayi enhle ye-cytoplasmic, futhi ivame ukusetshenziswa njengedayi ehlanganisa i-hematoxylin.

I-fuchsin eyisisekelo: i-fuchsin eyisisekelo iwudayi we-alkaline, osetshenziswa kabanzi ekukhiqizweni kwezinto eziphilayo futhi ungasetshenziswa ukungcolisa imicu ye-collagen kanye nemicu ye-elastic.

I-Crystal violet: I-Crystal violet iyidayi ye-alkaline, esetshenziswa kabanzi ku-cytology, histology kanye ne-bacteriology, iyibala elihle, elivame ukusetshenziselwa ukufaka ibala lenuzi.

I-Gentian violet: I-Gentian violet iyinhlanganisela yamadayi e-alkaline, ikakhulukazi ingxube ye-crystal violet ne-methyl violet, engasetshenziswa ngokushintshana ne-crystal violet uma kudingeka.

Lawa madayi adlala indima ebalulekile ekusikeni i-bioslicing, futhi ngezindlela ezahlukene zokufaka imibala kanye nokuhlanganiswa, angabonisa ngokucacile isakhiwo se-morphological samaseli nezicubu, anikeze ukwesekwa okubalulekile kobuchwepheshe ocwaningweni lwezinto eziphilayo kanye nolwezokwelapha.